Development of 31P Nuclear Magnetic Resonance Methods for the Study of Phosphate Metabolisms in E. coli and B. subtilis

Authors

  • Fabian M. Dayrit Department of Chemistry, Ateneo de Manila University, Loyola Heights, Quezon City
  • Emilyn Q. Espiritu Department of Chemistry, Ateneo de Manila University, Loyola Heights, Quezon City
  • Noreen Gonzalez Department of Chemistry, Ateneo de Manila University, Loyola Heights, Quezon City
  • Nina Rojas Department of Chemistry, Ateneo de Manila University, Loyola Heights, Quezon City
  • Jennifer T. Aguilan Department of Chemistry, Ateneo de Manila University, Loyola Heights, Quezon City
  • Antonio M. Basilio Department of Chemistry, Ateneo de Manila University, Loyola Heights, Quezon City
  • Edward T. Chainani Department of Chemistry, Ateneo de Manila University, Loyola Heights, Quezon City
  • Enrico Cruz Department of Chemistry, Ateneo de Manila University, Loyola Heights, Quezon City
  • Bernadette Matanguihan Department of Chemistry, Ateneo de Manila University, Loyola Heights, Quezon City

DOI:

https://doi.org/10.26534/kimika.v15i1.9-15

Keywords:

31P NMR method, phosphate metabolism, E. coli, B. subtilis

Abstract

31P NMR experiments were performed on Escherichia coli and Bacillus subtilis at various temperatures under aerobic and anaerobic conditions. The total soluble intracellular phosphate concentration was estimated to be 2 x 10^-17 mole/cell, while intracellular orthophosphate concentration was around 1 x 10^-17 mole/cell. Addition of glucose resulted in a general decrease in intracellular pH and was accompanied by the formation of sugar monophosphates. The concentrations of soluble intracellular phosphates, both inorganic and organic phosphates, were estimated by integration versus methylene diphosphonic acid (MDPA) standard. Although intracellular and extracellular orthophosphate could be observed, these appear to exchange rapidly on the NMR time scale.

Downloads

How to Cite

Dayrit, F. M., Espiritu, E. Q., Gonzalez, N., Rojas, N., Aguilan, J. T., Basilio, A. M., … Matanguihan, B. (1999). Development of 31P Nuclear Magnetic Resonance Methods for the Study of Phosphate Metabolisms in E. coli and B. subtilis. KIMIKA, 15(1), 9–15. https://doi.org/10.26534/kimika.v15i1.9-15

Issue

Vol. 15 No. 1 (1999)

Section

Research Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).