Preparation of Agarose by Electrolysis of Agar

Authors

  • Aileen M. Corpuz Institute of Chemistry and Marine Science Institute, University of the Philippines at Diliman, Quezon City
  • Florentino C. Sumera Institute of Chemistry and Marine Science Institute, University of the Philippines at Diliman, Quezon City

DOI:

https://doi.org/10.26534/kimika.v15i2.53-59

Keywords:

agarose, agar, electrolysis

Abstract

A method for the preparation of agarose from Difco bacto agar by means of electrolysis was developed. The method was established by analyzing the product for the following properties: gel strength, viscosity, gelling temperature, melting temperature. sulfate, 3,6-anhydrogalactose content, and electroendoosmotic value. The agarose produced has a gel strength of about 900 g/cm2 with a very low sulfate content of about 0.2% which makes it favorable for use in gel electrophoresis of proteins.

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How to Cite

Corpuz, A. M., & Sumera, F. C. (1999). Preparation of Agarose by Electrolysis of Agar. KIMIKA, 15(2), 53–59. https://doi.org/10.26534/kimika.v15i2.53-59

Issue

Vol. 15 No. 2 (1999)

Section

Research Articles

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