Elucidation of molecular functions of human tumor suppressor protein 101F6 by reconstitution into phospholipid bilayer nanodiscs

  • Mohammed El Behery Department of Chemistry, Graduate School of Science, Kobe University, Rokkodai-cho 1-1, Nada-ku, Kobe, Hyogo 657-8501
  • Akikazu Asada Department of Chemistry, Graduate School of Science, Kobe University, Rokkodai-cho 1-1, Nada-ku, Kobe, Hyogo 657-8501
  • Fusako Takeuchi IPHE, Kobe University, Tsurukabuto 1-2-1, Nada-ku, Kobe, Hyogo 657-8501
  • Tetsunari Kimura Department of Chemistry, Graduate School of Science, Kobe University, Rokkodai-cho 1-1, Nada-ku, Kobe, Hyogo 657-8501
  • Eri Chatani Department of Chemistry, Graduate School of Science, Kobe University, Rokkodai-cho 1-1, Nada-ku, Kobe, Hyogo 657-8501
  • Motonari Tsubaki Department of Chemistry, Graduate School of Science, Kobe University, Rokkodai-cho 1-1, Nada-ku, Kobe, Hyogo 657-8501
Keywords: cytochrome b561, nanodisc, ascorbate, membrane protein, electron transfer, apoptosis

Abstract

A candidate human tumor suppressor gene 101F6 product was expressed successfully in Pichia pastoris yeast cells. The purified 101F6 protein was successfully incorporated into phospholipid bilayer nanodiscs with different sizes by employing two reconstitution methods; self-assembly and reconstitution into the preformed empty nanodisc. The reconstituted 101F6 protein could be reduced with ascorbate quickly and was very stable even at ambient temperatures.

Published
2019-06-03
How to Cite
El Behery, M., Asada, A., Takeuchi, F., Kimura, T., Chatani, E., & Tsubaki, M. (2019). Elucidation of molecular functions of human tumor suppressor protein 101F6 by reconstitution into phospholipid bilayer nanodiscs. KIMIKA, 30(1), 1-3. Retrieved from https://kimika.pfcs.org.ph/index.php/kimika/article/view/267
Section
Research Articles