Binding of congo red and crystal violet with Î²-2-microglobulin amyloid fibril
Keywords:Î²-2-microglobulin, amyloid, crystal violet, congo red
AbstractDialysis-Related Amyloidosis (DRA) is a serious complication for patients undergoing long-term hemodialysis and severe renal failure. Its clinical manifestation (i.e., Carpal Tunnel Syndrome) may be associated mainly with fibril deposits of Î²-2-micro globulin (Î²2M) in the joints, kidney, and bone. Previous studies have been made to show the binding of dyes such as congo red (CR) and Thioflavin T to Î²2M fibrils. Since staining methods for the demonstration of Î²2M fibrils are limited, other dyes such as crystal violet (CV) may be developed as stains to elucidate amyloid fibrils. UV-Vis spectroscopy was used to demonstrate the shift in the maximum absorption wavelengths, which indicates binding, of the Î²2M seeded solutions examined. The use of UV-Vis spectroscopy as a technique for amyloid fibril demonstration serves as an advantage due to the instrument's availability in most laboratories. Î²2M solutions tested with CV demonstrates a shift (to shorter wavelengths) in the maximum absorption wavelengths. Thus, the development of these dyes as methods for amyloid elucidation will not only increase the variety of staining methods, but also will help design new therapeutic strategies to combat amyloidosis.
How to Cite
Panlilio, M. T. T., Quiming, N. S., Vergel, R., & Villanueva, J. A. (2004). Binding of congo red and crystal violet with Î²-2-microglobulin amyloid fibril. KIMIKA, 20(1), 13–16. https://doi.org/10.26534/kimika.v20i1.13-16
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).