Structure elucidation of β-stigmasterol and β-sitosterol from Sesbania grandifora [Linn.] Pers. and β-carotene from Heliotropium indicum Linn. by NMR spectroscopy

  • Jan-Michael C. Cayme Chemistry Department, De La Salle University, Manila
  • Consolacion Y. Ragasa Chemistry Department, De La Salle University, Manila
Keywords: Sesbania grandiflora (Linn.) Pers., Leguminosae, Heliotropium indicum Linn., Boraginaceae, β-stigmasterol, β-sitosterol, oleanolic acid, lutein, β-carotene, polyprenols, antimicrobial activity

Abstract

The flowers of Sesbania grandiflora (Linn.) Pers. afforded a 3:2 mixture of β-stigmasterol (1a) and β-sitosterol (1b), and oleanolic acid (2), while the leaves of Heliotropium indicum Linn. yielded β-carotene (3), lutein ( 4), a mixture of polyprenols (5), and a mixture of β-stigmasterol and β-sitosterol. The structures of 1a, 1b, and 3 were elucidated by extensive 1D and 2D NMR analyses. Antimicrobial activity tests on 3 indicated that it has high activity against Pseudomonas aeruginosa and Aspergillus niger, slight activity against Staphylococcus aureus, Escherichia coli, Candida albicans, and Trichophyton mentagrophytes, and inactive against Bacillus subtilis.
How to Cite
Cayme, J.-M. C., & Ragasa, C. Y. (1). Structure elucidation of β-stigmasterol and β-sitosterol from Sesbania grandifora [Linn.] Pers. and β-carotene from Heliotropium indicum Linn. by NMR spectroscopy. KIMIKA, 20(1), 5-12. https://doi.org/10.26534/kimika.v20i1.5-12
Section
Research Articles