Synthesis of metallothionein in the retina of cynomolgus monkeys

Authors

  • Marilou G. Nicolas Department of Physical Sciences & Mathematics, College of Arts and Sciences, University of the Philippines Manila, Ermita, Manila
  • Keiko Fujiki Department of Ophthalmology, Central Laboratory of Medical Sciences, Juntendo University School of Medicine, Tokyo
  • Kimei Murayama Division of Biochemical Analysis, Central Laboratory of Medical Sciences, Juntendo University School of Medicine, Tokyo
  • Michihiro Suzuki Corporation for Production and Research of Laboratory Primates
  • Noriko Shindo Division of Biochemical Analysis, Central Laboratory of Medical Sciences, Juntendo University School of Medicine, Tokyo
  • Yoshiidro Hotta Department of Ophthalmology, Central Laboratory of Medical Sciences, Juntendo University School of Medicine, Tokyo
  • Fumino Iwada Department of Ophthalmology, Central Laboratory of Medical Sciences, Juntendo University School of Medicine, Tokyo
  • Yasuhiro Yosidkawa Tsukuba Primate Center for Medical Sciences, National Institute of Health, Tsukuba
  • Fumaki Cho Tsukuba Primate Center for Medical Sciences, National Institute of Health, Tsukuba
  • Atsusid Kanai Department of Ophthalmology, Central Laboratory of Medical Sciences, Juntendo University School of Medicine, Tokyo

DOI:

https://doi.org/10.26534/kimika.v11i1.19-24

Keywords:

metallothionein, cynomolgus monkeys, retina, oxidative stress

Abstract

Metallothionein cDNA was detected in the retinas of cynomolgus monkeys (Macaca fascicularis) using RT-PCR. Sequencing of this cDNA showed almost a hundred percent sequence similarity with metallothionein cDNA from rhesus monkey kidneys. There was only a single base substitution (ACC - ACU) for Thr 14. A comparison of the amino acid sequence also showed a high percentage similarity with metallothionein class II isolated from other sources. Previous reports of several laboratories have implicated the induction of metallothionein synthesis as a response to oxidative stress which in turn is due to solar bombardment and phagocytosis of photoreceptors, particularly rod outer segments, by retinal pigment epithelium.

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How to Cite

Nicolas, M. G., Fujiki, K., Murayama, K., Suzuki, M., Shindo, N., Hotta, Y., … Kanai, A. (1995). Synthesis of metallothionein in the retina of cynomolgus monkeys. KIMIKA, 11(1), 19–24. https://doi.org/10.26534/kimika.v11i1.19-24

Issue

Vol. 11 (1995)

Section

Research Articles

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