Triacylglycerol Biosensor Based on Conducting Polymers

Authors

  • Amelita J. Bartolome Research Center for the Natural Sciences, University of Santo Tomas, Manila
  • Christina A. Binag Research Center for the Natural Sciences, University of Santo Tomas, Manila
  • Fortunato Sevilla III Research Center for the Natural Sciences, University of Santo Tomas, Manila

DOI:

https://doi.org/10.26534/kimika.v14i2.53-59

Keywords:

polypyrrole, galvanostatic polymerization, biosensor, triacylglycerol, conducting polymers

Abstract

A polypyrrole-based potentiometric triacylglycerol biosensor was developed and characterized. The biosensor was fabricated by the galvanostatic polymerization of polypyrrole (PPy) onto a platinum wire from a 0.1 M KHP buffer at pH 5.0 containing 0.1 M monomer and 3 mg lipase (Lip). The biosensor exhibited high linearity (r = 0.9980), with a working range to triacetin of 25 to 150 mM. XPS spectra of the biosensor verified the presence of the enzyme with the sulfuhydryl groups. With SEM, black polymer film showed nonuniform, well-adhering fern-like surface morphology.

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How to Cite

Bartolome, A. J., Binag, C. A., & Sevilla III, F. (1998). Triacylglycerol Biosensor Based on Conducting Polymers. KIMIKA, 14(2), 53–59. https://doi.org/10.26534/kimika.v14i2.53-59

Issue

Vol. 14 No. 2 (1998)

Section

Research Articles

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