Optical Fiber Chemiluminescence Sensor for Iron(II) Ion Based on Immobilized Luminol

Authors

  • Arthur A. Alipao Research Center for the Natural Sciences, University of Santo Tomas, España, Manila
  • Fortunato Sevilla III Research Center for the Natural Sciences, University of Santo Tomas, España, Manila

DOI:

https://doi.org/10.26534/kimika.v15i1.21-25

Keywords:

Chemiluminescence sensor, optical fiber sensor, iron(ll) sensor, immobilized luminol reagents

Abstract

A chemiluminescence (CL) sensor for iron(II) was developed based on the catalytic action of the analyte on the CL reaction between luminol and oxygen. The reagents were immobilized on a cellulose membrane and set on a reaction cell which was coupled by means of an optical fiber to a spectrofluorometer. The concentration of iron(ll) was quantified by measuring the intensity of the light generated from the CL reaction. The response of the sensor system was rapid and highly reproducible. Good sensitivity was displayed by the sensor system over five orders of magnitude of iron(ll) ion concentration. The calibration curve consisted of two portions: (1) a linear range at lower concentrations (7.5 x 10^-7 M to 1.0 x 10^-4 M) exhibiting a positive slope arising from a catalytic action, and (2) a linear range at higher concentrations (5.0 x 10^-2 M to 2.5 x 10^-4 M) wherein the slope is negative due to an inhibitory action of iron(ll) on the CL reaction. The sensor system was highly selective for iron(II) ions.

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How to Cite

Alipao, A. A., & Sevilla III, F. (1999). Optical Fiber Chemiluminescence Sensor for Iron(II) Ion Based on Immobilized Luminol. KIMIKA, 15(1), 21–25. https://doi.org/10.26534/kimika.v15i1.21-25

Issue

Vol. 15 No. 1 (1999)

Section

Research Articles

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