A Molecularly Imprinted Polymer Stationary Phase for Chiral Separation of Ofloxacin by Thin Layer Chromatography

  • Teresita A. Tamayo College of Arts and Sciences, Bulacan State University, Malolos, Bulacan
  • Florian R. del Mundo Institute of Chemistry, College of Science, University of the Philippines, Diliman, Quezon City
  • Leonorina G. Cada Institute of Chemistry, College of Science, University of the Philippines, Diliman, Quezon City
  • Ernesto C. Dela Cruz Industrial Technology and Development Institute, Department of Science and Technology, Bicutan, Taguig
Keywords: ofloxacin, levofloxacin, molecular imprinting, thin layer chromatography, chiral separation

Abstract

A molecular imprinting procedure was adopted to prepare a highly crosslinked polymer for the racemic resolution of ofloxacin, a wide spectrum quinolone anti infective agent. A combination of acidic and basic functional monomers, acrylic acid and 4-vinyl pyridine at different proportions, were copolymerized with ethylene glycol dimethacrylate by thermal initiation. These polymers, after drying, sizing, and removal of print molecule, were used in a batch binding experiment and made into thin layer chromatography (TLC) plates for thin layer chromatographic procedure to examine their enantioselectivity for levofloxacin or S-ofloxacin. The results showed that polymer P33 has comparatively higher binding capacity and selectivity than the other polymers as far as S-ofloxacin is concerned. This was confirmed by CD-ORD results. This preliminary report demonstrates a novel and promising family of stationary phase (based on predetermined selectivity) for use not only for solid phase extraction but also for liquid chromatography and capillary electrophoresis.
How to Cite
Tamayo, T. A., del Mundo, F. R., Cada, L. G., & Dela Cruz, E. C. (1). A Molecularly Imprinted Polymer Stationary Phase for Chiral Separation of Ofloxacin by Thin Layer Chromatography. KIMIKA, 17(2), 53-56. https://doi.org/10.26534/kimika.v17i2.53-56
Section
Research Articles