Supercritical fluid-extraction of polynuclear aromatic hydrocarbons from <75-pM wet-sieved sediments: A method development and validation study

Authors

  • Alma Oberes-Gonzales Department of Chemistry, Mindanao State University-Iligan Institute of Technology, Iligan City
  • Mario B. Capangpangan Department of Chemistry, Mindanao State University-Iligan Institute of Technology, Iligan City

DOI:

https://doi.org/10.26534/kimika.v18i2.33-40

Keywords:

PAHs, sediments, supercritical, SFE, HPLC, method development, method validation

Abstract

An analytical method for the isolation, detection, and quantitation of selected polynuclear aromatic hydrocarbons (PARs) namely naphthalene, phenanthrene, fluoranthene, and pyrene from <75-µm wet-sieved and closed-jar dried sediment samples has been developed and validated. Supercritical Fluid Extraction (SFE) and High Performance Liquid Chromatography (HPLC) were utilized as the sample preparation and analyte detection techniques, respectively. The developed method was evaluated in comparison with Soxhlet extraction as the reference method.In the method development phase, the SFE method achieved an overall accuracy (% recovery) of 94.59%, with an overall precision of 1.02% RSD, while Soxhlet extraction had an overall accuracy of 67.99%, and an overall precision of 13.89% RSD. These preliminary results showed that SFE method has much better performance than Soxhlet extraction.Moreover, in the method validation phase, the developed SFE method achieved an overall accuracy of 93.94%, with an overall precision of 3.47% RSD, while Soxhlet extraction had an overall accuracy of 68.66%, and an overall precision of 23.9 3% RSD. These results indicate that SFE method is more accurate and more precise than Soxhlet extraction, thus, proving it was highly acceptable for application purposes. Therefore, the developed SFE method has been validated.

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How to Cite

Oberes-Gonzales, A., & Capangpangan, M. B. (2002). Supercritical fluid-extraction of polynuclear aromatic hydrocarbons from <75-pM wet-sieved sediments: A method development and validation study. KIMIKA, 18(2), 33–40. https://doi.org/10.26534/kimika.v18i2.33-40

Issue

Vol. 18 No. 2 (2002)

Section

Research Articles

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