Fiber-optic biosensors based on covalently immobilized enzymes and thymol blue

  • Roberto Andres University of Santo Tomas, Manila
  • Fortunato Sevilla III University of Santo Tomas, Manila
  • Ramaier Narayanaswamy University of Manchester Institute of Science and Technology, Manchester
Keywords: fiber optic biosensors, thymol blue, immobilized enzymes, urease-based biosensors, lipase-based biosensors, acetyl cholinesterase-based biosensors

Abstract

Fiber optic biosensors based on immobilized enzymes (urease, lipase and acetyl cholinesterase) in conjunction with immobilized thymol blue are described, and their uses in the detection of a wide range of analytes are discussed. The bioactive materials of the optical biosensors consist of enzymes covalently attached to preactivated controlled pore glass mixed with thymol blue, also covalently bound to similar matrix. A thin layer of the glass bead mixture was spread at the tip of a bifurcated fiber-optic sensor head to construct the sensor, which was then integrated with a flow-cell. The biosensors exhibit responses to different types of analytes (substrates, metal ions and pesticides), which are highly reproducible and linear over a range of analyte concentrations.
Published
2003-06-01
How to Cite
Andres, R., Sevilla III, F., & Narayanaswamy, R. (2003). Fiber-optic biosensors based on covalently immobilized enzymes and thymol blue. KIMIKA, 19(1), 29-34. https://doi.org/10.26534/kimika.v19i1.29-34
Section
Research Articles