Comparative Gene Expression Profiling in Cultured Cell Lines after Treatment with Wasabi-derived Isothiocyanates
Isothiocyanate (ITC) is a small but reactive organo-sulfur containing group of compound present in cruciferous vegetables. Compendium of evidence indicated that ITCs exhibit multiple biological activities, but the exact molecular mechanisms are not yet clear. Therefore, this study was designed to compare the genome-wide gene expression profiles in hepatic and neuron cells following wasabi-derived ITCs treatment using microarray technology. Cells were treated with wasabi-derived ITCs, sulforaphane (SFN), 6-(methylsulfinyl)hexyl isothiocyanate (6-MSITC) and 6-(methylthio)hexyl isothiocyanate (6-MTITC), for 9 h and was followed by DNA microarray analyses using HG-U133 plus 2.0 oligonucleotide array. Selected gene products were confirmed by real-time PCR, and functional subsets of genes and biologically significant network were identified using Ingenuity Pathway Analysis. Results showed that 6-MTITC was the most potent inducer of gene expressions changes in HepG2 cells, whereas 6-MSITC was the most effective inducer in IMR-32 cells. Despite this cell-type response discrepancies, 6-MSITC came up as the strongest inducer of antioxidant-associated genes, via the regulation of the Nrf2-mediated pathway. These results combined with the varying induction level data of other Wasabi-derived ITCs form the basis for further studies to assess the possible therapeutic effect of combined Wasabi-derived ITCs treatment. Altogether, this study provided comprehensive information on how structural differences of Wasabi-derived ITCs contribute to its efficacy and impact specific targets.
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