Pulse Voltammetric Detection of Proteins Using a Polypyrrole/Methyl Orange Coated Platinum Electrode
Keywords: polypyrrole-based sensor, proteins, pulse voltammetry, flow injection
AbstractThis study has shown that the incorporation of a methyl orange dye into a conducting polymer-coated platinum electrode enables sensitive responses to electroinactive proteins obtained using normal pulse voltammetry (NPV). Using 1 M Na2S04 (pH 6.5) as supporting electrolyte, the order of decreasing calibration sensitivity of polypyrrole/methyl orange (PMO)-coated Pt electrode response to proteins is lactalbumin(LAC) > myoglobin(MYO) > ovalbumin(OVA) > bovine serum albumin(BSA). The potential application of the PMO-coated Pt electrode for routine electroanalysis of proteins was also studied using flow injection analysis (FIA) with pulse amperometric detection. The optimum FIA response of the different proteins studied were obtained using a carrier stream whose pH is approximately equai to their isoelectric point. The FIA method gave reproducible protein responses ranging from 0.2 -14% RSD. The estimated detection limit of the proteins studied are BSA (5.9 x 10^-5 µM), lv1YO (1.1 x 10^-3 µM), and OVA (89 µM).
How to Cite
del Mundo, F. R., Oliva, M. dL., & Yaya, R. S. (2006). Pulse Voltammetric Detection of Proteins Using a Polypyrrole/Methyl Orange Coated Platinum Electrode. KIMIKA, 22(1), 23-31. https://doi.org/10.26534/kimika.v22i1.23-31
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