Identification of Glucoamylase cDNA Sequence of Saccharomycopsis (Syn. Endomycopsis) bubodii 2066
Keywords: glucoamylase, cDNA, primer walking, Saccharomycopsis bubodii, Saccharomycopsis fibuligera, sago starch
AbstractSaccharomycopsis (Syn. Endomycopsis) bubodii 2066 is an isolate from bubod, a starter used in making rice wine in northern Philippines. We have shown that the yeast has amylolytic activity on raw sago starch. In our attempt to identify the putative raw starch-digesting amylase in S. bubodii, we determined the cDNA sequence of a glucoamylase gene. One primer pair that was designed based on a glucoamylase of Saccharomycopsis fibuligera HUT7212 (GLU1, NCBI Accession Number L25641.1) produced a sequence of 1234 base pairs. To obtain a wider coverage, a primer walking strategy was carried out using four primer pairs designed based on GLU1 gene. The generated sequence of 1535 base pairs shows 98.7 to 100% homology when aligned with glucoamylase genes from four strains of S. fibuligera suggesting that this glucoamylase is highly conserved between the Saccharomycopsis species. This work further reports a gene sequence of glucoamylase derived from Philippine-isolated yeast. The sequence is deposited in GenBank and assigned the accession number KP068007.1. The gene may be heterologously expressed in Saccharomyces cerevisiae for possible utilization in the direct conversion of raw sago starch to bioethanol.
How to Cite
Tolentino, J. H. G., Labrador, K. L., Fronteras, J. P., Bullo, L. L. R., Cancio, L. P. M., Añonuevo, J. J. J., Eleria, G. P. G., & Novero, A. U. (2016). Identification of Glucoamylase cDNA Sequence of Saccharomycopsis (Syn. Endomycopsis) bubodii 2066. KIMIKA, 27(2), 14-27. https://doi.org/10.26534/kimika.v27i2.14-27
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).