The triacylglycerols from microbial lipase-catalyzed reaction of coconut oil and oleic acid

Authors

  • Eduardo G. Lorica San Miguel Brewery, Polo, Bulacan
  • Rosenda G. Lorica Institute of Chemistry, University of the Philippines, Diliman, Quezon City
  • Florentino C. Sumera Institute of Chemistry, University of the Philippines, Diliman, Quezon City

DOI:

https://doi.org/10.26534/kimika.v12i1.15-23

Keywords:

triacylglycerols, oleyl acylglycerols, coconut oil, oleic acid, microbial lipase interesterification, transesterification, acidolysis, Candida cylindracea

Abstract

The product triacylglycerols from the reaction of coconut oil and oleic acid using microbial lipase from Candida cylindracea as catalyst were analyzed by reversed phase HPLC on C18 column. A peak by peak analysis of the HPLC chromatogram of the triacylglycerol product from lipase-catalyzed interesterification reaction between coconut oil and oleic acid is herein presented. Analysis of the column fractions revealed that oleic acid has been introduced by substitution into the triacylglycerols of coconut oil. Individual column fractions after their saponification and determination of their fatty acid composition showed the presence of mixed tiacylglycerols. The retention times of these mixed triacylglycerols were determined by comparison with those of triacylglycerols microbially synthesized by acidolysis of simple triacylglycerols with oleic acid or by esterification of fatty acids (present in coconut oil) with oleic acid and glycerol. From correlation of carbon numbers with retention times of simple triacylglycerols, the retention times of the mixed triacylglycerols were extrapolated. It was deduced from this correlation that oleyl acylglycerols coeluted with saturated triacylglycerols of lower carbon numbers.

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How to Cite

Lorica, E. G., Lorica, R. G., & Sumera, F. C. (1996). The triacylglycerols from microbial lipase-catalyzed reaction of coconut oil and oleic acid. KIMIKA, 12(1), 15–23. https://doi.org/10.26534/kimika.v12i1.15-23

Issue

Vol. 12 (1996)

Section

Research Articles

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