A Molecularly Imprinted Polymer Stationary Phase for Chiral Separation of Ofloxacin by Thin Layer Chromatography

Authors

  • Teresita A. Tamayo College of Arts and Sciences, Bulacan State University, Malolos, Bulacan
  • Florian R. del Mundo Institute of Chemistry, College of Science, University of the Philippines, Diliman, Quezon City
  • Leonorina G. Cada Institute of Chemistry, College of Science, University of the Philippines, Diliman, Quezon City
  • Ernesto C. Dela Cruz Industrial Technology and Development Institute, Department of Science and Technology, Bicutan, Taguig

DOI:

https://doi.org/10.26534/kimika.v17i2.53-56

Keywords:

ofloxacin, levofloxacin, molecular imprinting, thin layer chromatography, chiral separation

Abstract

A molecular imprinting procedure was adopted to prepare a highly crosslinked polymer for the racemic resolution of ofloxacin, a wide spectrum quinolone anti infective agent. A combination of acidic and basic functional monomers, acrylic acid and 4-vinyl pyridine at different proportions, were copolymerized with ethylene glycol dimethacrylate by thermal initiation. These polymers, after drying, sizing, and removal of print molecule, were used in a batch binding experiment and made into thin layer chromatography (TLC) plates for thin layer chromatographic procedure to examine their enantioselectivity for levofloxacin or S-ofloxacin. The results showed that polymer P33 has comparatively higher binding capacity and selectivity than the other polymers as far as S-ofloxacin is concerned. This was confirmed by CD-ORD results. This preliminary report demonstrates a novel and promising family of stationary phase (based on predetermined selectivity) for use not only for solid phase extraction but also for liquid chromatography and capillary electrophoresis.

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How to Cite

Tamayo, T. A., del Mundo, F. R., Cada, L. G., & Dela Cruz, E. C. (2001). A Molecularly Imprinted Polymer Stationary Phase for Chiral Separation of Ofloxacin by Thin Layer Chromatography. KIMIKA, 17(2), 53–56. https://doi.org/10.26534/kimika.v17i2.53-56

Issue

Vol. 17 No. 2 (2001)

Section

Research Articles

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