Partial Characterization of Glycans from Daedalea quercina (L) Fr.
DOI:
https://doi.org/10.26534/kimika.v26i2.51-58Keywords:
Daedalea quercina, glycans, HPAEC-PAC, MALDI-TOF MS, monosaccharide analysisAbstract
Heteroglycans were extracted and isolated from the fruiting bodies of wild mushroom Daedalea quercina (L) Fr. These were obtained from sequential extraction with hot water, 1% Ammonium oxalate then 5% Sodium hydroxide solution followed by ethanol or acidic precipitation affording crude extracts which were further purified by dialysis membrane (MWCO 12 400). Â Separation by charge and size were done using DEAE-cellulose column and Sephadex G-100 or Sepharose CL-4B. This resulted to water soluble neutral (W1P-1A) and acidic (W1P-2A) glycan isolates, ammonium oxalate soluble (A1P-1) and two alkali soluble (N1P-1 and N1P-2) glycan isolates. Characterization of the hydrolyzed isolates using HPAEC-PAD and MALDI TOF MS showed glucan-rich heteroglycans (W1P-1A and W1P-2A); glucan and mannan rich heteroglycan, (N1P-1) and galactan-rich heteroglycan (A1P-1). The latter being the first reported galactan-rich glycan found in ammonium oxalate mushroom extract. The hydrolysates had approximately 4-5 degree of polymerization with molecular weights ranging from 689.680 to 851.788 Da. The hydrolysate of isolate N1P-2 is a glucan-rich heteroglycan which also contain N-acetyl glucosamine units. It is composed of polymers with 4-5 hexose units as well as N-acetyl glucosamine containing polymers of 6-8 hexose units with molecular weight range of 689.799 to 851.915 Da and 1055.718 to 1379.584 Da, respectively.Downloads
Published
2016-02-26
How to Cite
Clerigo, M. M., Yang, W. B., & Paano, A. M. C. (2016). Partial Characterization of Glycans from Daedalea quercina (L) Fr. KIMIKA, 26(2), 51–58. https://doi.org/10.26534/kimika.v26i2.51-58
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Research Articles
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