In situ Protein Reactivity Profile of Intracellular Protein Targeted by Pb, Cd in Heavy-Metal-Tolerant Marine Sediment Bacterial Isolated from Illigan Bay

Authors

  • Mannix P. Balanay Department of Chemistry, College of Arts and Sciences, Silliman University, Dumaguete City
  • Mrynna S. Mahinay Department of Chemistry, College of Science and Mathematics, Mindanao State University - Iligan Institute of Technology, Iligan City
  • Arvin B. Balala Department of Chemistry, College of Science and Mathematics, Mindanao State University - Iligan Institute of Technology, Iligan City
  • Anita P. Rivera Department of Chemistry, College of Science and Mathematics, Mindanao State University - Iligan Institute of Technology, Iligan City
  • Alexes C. Daquinag Molecular Physiology & Biophysics, Baylor College of Medicine, Houston, Texas

DOI:

https://doi.org/10.26534/kimika.v22i2.87-95

Keywords:

bioremediation, in situ protein profile, metallothionein, metal-binding motif, biosorption efficiency

Abstract

The current research has shown promising potential for the application of the endemic, indigenous, non-pathogenic and heavy-metal-tolerant marine sediment bacteria isolated from Iligan Bay in the bioremediation of solid waste or wastewater contaminated with the heavy metals Pb and Cd. Optical density, wet-weight differential, paper disc diffusion, biosorption efficiency, quantification of the metallothionein, and in situ protein reactivity profile using the 10% SDS-PAGE have shown a potential of metal uptake by the bacteria. Reactivity with the target protein having the MW of ~39 kDa formed the complex having a MW of ~42 kDa.

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Published

2006-11-01

How to Cite

Balanay, M. P., Mahinay, M. S., Balala, A. B., Rivera, A. P., & Daquinag, A. C. (2006). In situ Protein Reactivity Profile of Intracellular Protein Targeted by Pb, Cd in Heavy-Metal-Tolerant Marine Sediment Bacterial Isolated from Illigan Bay. KIMIKA, 22(2), 87–95. https://doi.org/10.26534/kimika.v22i2.87-95

Issue

Vol. 22 No. 2 (2006)

Section

Research Articles

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